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Society should push authorities to quickly adopt successfully validated techniques, while realizing that pushing for adoption without full validation could endanger human health. Despite the inherent limitations of some non-animal tests, they are still useful for pre-screening compounds before the animal-testing stage, which would therefore reduce rather than replace the number of animals used. An example of this is the Ames test, which uses strains of the bacterium Salmonella typhimurium to determine whether chemicals cause mutations in cellular DNA. This and other tests are already widely used as pre-screens to partly replace rodent testing for cancer-causing compounds. Unfortunately, the in vitro tests can produce false results, and tend to be used more to understand the processes of mutagenicity and carcinogenicity than to replace animal assays. However, there are moves to replace the standard mouse carcinogenicity assay with other animal-based tests that cause less suffering because they use fewer animals and do not take as long. Although animals cannot yet be completely replaced, it is important that researchers maximize refinement and reduction. Microdosing is an exciting new technique for measuring how very small doses of a compound move around the body. In principle, it should be possible to use this method in humans and therefore to reduce the number of animals needed to study new compounds; however, it too has limitations. By its very nature, it cannot predict toxicity or side effects that occur at higher therapeutic doses. The claim by anti-vivisection campaigners that research into replacements is neglected merely reflects their ignorance. Good science and good experimental design also help to reduce the number of animals used in research as they allow scientists to gather data using the minimum number of animals required. However, good science also means that a sufficient number must be used to enable precise statistical analysis and to generate significant results to prevent the repetition of experiments and the consequent need to use more animals. In 1998, FRAME formed a Reduction Committee, in part to publicize effective reduction techniques. The data collected by the Committee so far provides information about the overall reduction in animal usage that has been brought about by the efforts of researchers worldwide (FRAME Reduction Committee, 2005). For example, screening potential anti-cancer drugs uses the so-called hollow-fibre system, in which tumour cells are grown in a tube-like polymer matrix that is implanted into mice. Drugs are then administered, the tubes removed and the number of cells determined. This system has increased the amount of data that can be obtained per animal in some studies and has therefore reduced the number of mice used (Double, 2004). In neuroscience, techniques such as cooling regions of the brain instead of removing subsections, and magnetic resonance imaging, have both helped to reduce the number of laboratory animals used (Royal Society, 2004). The benefits of animal research have been enormous and it would have severe consequences for public health and medical research if it were abandoned. Matching the number of animals generated from breeding programmes to the number of animals required for research has also helped to reduce the number of surplus animals. For example, the cryopreservation of sperm and oocytes has reduced the number of genetically modified mice required for breeding programmes (Robinson et al , 2003); mice lines do not have to be continuously bred if they can be regenerated from frozen cells when required. Although animals cannot yet be completely replaced, it is important that researchers maximize reduction and refinement. Sometimes this is achieved relatively easily by improving animal husbandry and housing, for example, by enriching their environment. These simple measures within the laboratory aim to satisfy the physiological and behavioural needs of the animals and therefore maintain their well-being. Another important factor is refining the experimental procedures themselves, and refining the management of pain. An assessment of the method of administration, the effects of the substance on the animal, and the amount of handling and restraint required should all be considered. Furthermore, careful handling of the animals, and administration of appropriate anaesthetics and analgesics during the experiment, can help to reduce any pain experienced by the animals, assignment maker uk. This culture of care is achieved not only through strict regulations but also by ensuring that animal technicians and other workers understand and adopt such regulations. Therefore, adequate training is an important aspect of the refinement of animal research, and should continually be reviewed and improved.https://classroom.itsekirichatgroup.com/groups/steps-in-writing-a-paper-steps-in-writing-a-documented-essay/
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